Andrea Wiget

Nuclear Magnetic Resonance Spectroscopy: fragment based screen using 19F and 1H ligand observed techniques, protein and protein ligand characterization by 1D and 2D NMR spectroscopy Process automation and harmonization by developing a Filemaker based database together with external partners Unix based system software Other biophysical analytic techniques: Surface Plasmon Resonance, Isothermal Calorimetry, Differential Scanning Fluorimetry Presenting data at project meetings

Biophysical analytics: Surface plasmon resonance: development of new assays, performance of different interaction analysis, data evaluation Analytical ultracentrifugation Fluorescence titration Mentoring of trainees Writing reports and presenting results at project meetings

Investigation of the acid stress response of a special E. coli strain: Cultivation of bacteria, enzyme induction Establishment of assays for specific enzyme activity 2D-electrophoresis and data analysis

GC-MS and HPLC analysis of pesticide and antibiotic residues in vegetables and meat, respectively Wet chemical methods

Method validation for the separation of chiral compounds (in particular alpha-Tocopherol) using HPLC und CE