Dipl.-Ing. Hamid Rasoulimehrabani

In my studies at the University of Vienna, we investigate the selectivity of prebiotics in human gut microbiota in vitro models for evaluating their functionality in microbial ecology. We are using different tools and methods such as a fluorescence-based single-cell activity measure [biorthogonal non-canonical amino acid-tagging (BONCAT)] combined with fluorescence-activated cell sorting (FACS), Raman microspectroscopy with Raman activated cell sorting and microfluidics to determine which microorganisms are metabolically active under the presence of the prebiotics. I was a project Assistant at the Institute of Chemical Technologies and Analytics, TU Wien, Austria. Raman imaging of PMMA particles in an aqueous environment was successfully performed using two different Raman set-ups: the confocal Raman microscope (Horiba Jobin-Yvon LabRAM 800HR-633nm) and a Raman probe optimized for process monitoring (Kaiser Optical Systems RXN-1-785 nm).