Ludmila Friesen
Angestellt, Clinical Research Associate, Roche Diagnostics International Ltd
Rotkreuz, Schweiz
Werdegang
Berufserfahrung von Ludmila Friesen
Bis heute 4 Jahre und 6 Monate, seit Jan. 2020
Clinical Research Associate
Roche Diagnostics International Ltd
Bis heute 9 Jahre und 1 Monat, seit Juni 2015
Clinical Research Associate
Kelly Services / Roche Diagnostics AG
1 Jahr und 8 Monate, Mai 2013 - Dez. 2014
Scientific assistant
Institute for Applied Medical Engineering, Aachen
MicroBioMed Project, Research assistant •Production and purification of recombinant antigens, monoclonal antibodies (IMAC, Protein A/G Chromatography) •Characterisation of proteins and antibodies (ELISA, flow cytometry, Immunohistochemistry, Western Blot, PAGE, receptor internalisation studies, SNAP-tag technology) •Cell culture •ELISA •Immunisation/blood collection from mice, titer •DNA/RNA purification, cDNA Synthesis, PCR, cloning •Supervision of students
8 Monate, Sep. 2012 - Apr. 2013
Master Student
Fraunhofer IME Aachen, Pflanzenbiotechnologie
Master Thesis in the Plant Biotechnology Group: work on the immobilisation of Malaria vaccine candidates on the surface of CPMV empty virus like particles (eVLPs) • Production of Malaria vaccine candidates and CPMV eVLPs by transient expression in N. benthamiana • Chemical conjugation of vaccine candidates on the surface of the eVLPs • Evaluation of conjugation efficiency by agarose gel electrophoresis of proteins, gel filtration chromatography, Western blot, PAGE
6 Monate, März 2012 - Aug. 2012
Research Internship
John Innes Centre, Norwich, UK
Work on the development of Schmallenberg Virus Detection Assay in cooperation with The Pirbright Institute • production of viral antigens (Schmallenberg virus, Bluetongue virus (BTV)) in N. benthamiana • production of BTV eVLPs in plant: purification by density gradient ultracentrifugation, integrity investigation by TEM)
9 Monate, Feb. 2011 - Okt. 2011
Graduate Research Assistant
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME)
Work on the Wine Yeast Project in cooperation with Winery in Salgesh and Fraunhofer Institute in Schmallenberg • Evaluation of the native antigen recognition on the yeast cell surface in flow cytometry and fluorescence microscopy • Development of a yeast detection quick-test (evaluation of materials, geometry calculation, statistical evaluation)
4 Monate, Sep. 2010 - Dez. 2010
Graduate Research Assistant
Helmholtz-Institut - RWTH Aachen und Universitätsklinikum Aachen
•Production of HCV Antigens in E. coli (periplasmic expression) and HEK293T cells •Optimisation of Expression conditions and IMAC-purification conditions •Molecular cloning
7 Monate, März 2010 - Sep. 2010
Bachelorand
Fraunhofer IME Aachen, Pharmazeutische Produktentwicklung
Worke on the development of an immunological quick-test for the detection of wild yeasts in spontaneous wine fermentation • Purification of polyclonal antibodies from rabbit sera (Protein G, Äkta purifier) • Characterisation of polyclonal antibodies (PAGE, native/reducing; Western blot; titer determination) • Reduction of antibody cross-reactivity by pre-adsorption • Set-up and evaluation of a sandwich ELISA
1 Jahr und 5 Monate, Dez. 2006 - Apr. 2008
Specialized Shop Assistants
Roger´s Sportswear
Ausbildung von Ludmila Friesen
3 Monate, März 2015 - Mai 2015
Weiterbildung Klinische Studien
mibeg-Institut Medizin
5 Jahre und 7 Monate, Okt. 2007 - Apr. 2013
Molecular and Applied Biotechnology
RWTH Aachen University
Red Biotechnology: cell biology, immunology, protein chemistry, signal transduction, biomaterials and bioactive peptides Molecular and Industrial Biotechnology, Bioprocessing
11 Monate, Sep. 2002 - Juli 2003
Bioecology
Orenburg State Agricultural University (OSAU)
Zoology, Botany, Anatomy and Evolutionary Morphology, Geology, Inorganic and Analytical Chemistry General education: Philosophy, Economy, Geography, Mathematics
Sprachen
Englisch
Fließend
Deutsch
Muttersprache
Russisch
Muttersprache
Französisch
Grundlagen