Yasaman Sotoodeh

Title: Molecular characterization of two T-DNA insertion lines for the putative splicing factor MERISTEM DEFECTIVE (MDF) in Arabidopsis thaliana. In my master thesis, I studied the MDF gene and two mutants of this gene again in Arabidopsis thaliana. During my thesis, I analyzed the changes of expression of DNA repair genes in this gene’s mutations, tried to understand the role of MDF in splicing, and also analyzed the phenotype of mutation lines as well as tried to cure the phenotype changes

Title: Analysis of mutant mipp-4 PP7 type phosphatase in Arabidopsis thaliana PP7 homolog MIPP has been identified as an interaction partner for MAIL1, their mutants both show the short root and cell death in the RAM. I have analyzed the difference between mutant lines of MIPP for the expression level of MIPP, cell death, and genome instability in RAM. My results were helpful for poblish: PP7L is essential for MAIL1-mediated transposable element silencing and primary root growth.

Title: Generation of NHL4/6/25-specific promoter-reporter (GusA) in Arabidopsis thaliana using gateway cloning and phenotype analysis of nhl4/6/25 single mutants in the response to ABA. I have worked on mutant lines of NHL gene in Arabidopsis thaliana, The purposes of the project were to determine whether the NHL has the same function as NDR1 and HIN1 and also to analyze the phenotype by abscisic acid treatment.

During my Master program, I worked independently in different Molecular labs and analyzed the data on my own, therefore my laboratory and research skills are well-developed. I mostly worked on genome, RNA, and protein, and learned the tools for laboratory works and analyzing the data.

During my bachelor program, I had theoretical and laboratory of Biochemistry, Molecular and Cellular Biology, Genetics, virology, embryology, bacteriology, and many other courses.